B3 Science is organised into the following five biosecurity themes:

This theme is the basis of most, if not all, strategic planning around biosecurity. Research in this theme seeks to identify border biosecurity hazards, assess the associated risks and determine optimal mitigation solutions.
Work in this theme supports activities aimed at understanding and reducing biosecurity risks associated with cross-border movement of goods and people.
This research involves determining the identity of exotic pests and diseases, identifying the causes of disease symptoms and evaluating any forensic information critical to response decisions.
This theme includes work on small traps and new lures to detect insect invaders earlier, surveillance models to design better surveillance programmes and methods to detect new harmful pathogens cost-effectively.
This work focuses on the impacts of rapidly increasing tourism and trade activity, which means that new organisms are being detected on a weekly basis, some of which could have significant economic impact on New Zealand.